A universal protocol for the combined isolation of metabolites, DNA, long RNAs, small RNAs, and proteins from plants and microorganisms.
Identifieur interne : 002385 ( Main/Exploration ); précédent : 002384; suivant : 002386A universal protocol for the combined isolation of metabolites, DNA, long RNAs, small RNAs, and proteins from plants and microorganisms.
Auteurs : Luis Valledor [République tchèque] ; M Nica Escand N ; M Nica Meij N ; Ella Nukarinen ; María Jesús Ca Al ; Wolfram WeckwerthSource :
- The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2014.
Descripteurs français
- KwdFr :
- ADN des plantes (isolement et purification), ARN des plantes (isolement et purification), Arabidopsis (génétique), Arabidopsis (métabolisme), Biologie des systèmes (méthodes), Chlamydomonas reinhardtii (génétique), Chlamydomonas reinhardtii (métabolisme), Génomique (méthodes), Métabolomique (méthodes), Pinus (génétique), Pinus (métabolisme), Plantes (génétique), Plantes (métabolisme), Populus (génétique), Populus (métabolisme), Protéines végétales (isolement et purification), Protéomique (méthodes), Reproductibilité des résultats (MeSH).
- MESH :
- génétique : Arabidopsis, Chlamydomonas reinhardtii, Pinus, Plantes, Populus.
- isolement et purification : ADN des plantes, ARN des plantes, Protéines végétales.
- métabolisme : Arabidopsis, Chlamydomonas reinhardtii, Pinus, Plantes, Populus.
- méthodes : Biologie des systèmes, Génomique, Métabolomique, Protéomique.
- Reproductibilité des résultats.
English descriptors
- KwdEn :
- Arabidopsis (genetics), Arabidopsis (metabolism), Chlamydomonas reinhardtii (genetics), Chlamydomonas reinhardtii (metabolism), DNA, Plant (isolation & purification), Genomics (methods), Metabolomics (methods), Pinus (genetics), Pinus (metabolism), Plant Proteins (isolation & purification), Plants (genetics), Plants (metabolism), Populus (genetics), Populus (metabolism), Proteomics (methods), RNA, Plant (isolation & purification), Reproducibility of Results (MeSH), Systems Biology (methods).
- MESH :
- chemical , isolation & purification : DNA, Plant, Plant Proteins, RNA, Plant.
- genetics : Arabidopsis, Chlamydomonas reinhardtii, Pinus, Plants, Populus.
- metabolism : Arabidopsis, Chlamydomonas reinhardtii, Pinus, Plants, Populus.
- methods : Genomics, Metabolomics, Proteomics, Systems Biology.
- Reproducibility of Results.
Abstract
Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as a major step for multilevel data integration strategies in systems biology. While extracting proteins and DNA, this protocol also allows the separation of metabolites into polar and lipid fractions, as well as RNA fractionation into long and small RNAs, thus allowing a broad range of transcriptional studies. The isolated biomolecules are suitable for analysis with different methods that range from electrophoresis and blotting to state-of-the-art procedures based on mass spectrometry (accurate metabolite profiling, shot-gun proteomics) or massive sequencing technologies (transcript analysis). The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues.
DOI: 10.1111/tpj.12546
PubMed: 24804825
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24804825</PMID><DateCompleted><Year>2015</Year><Month>02</Month><Day>24</Day></DateCompleted><DateRevised><Year>2014</Year><Month>06</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1365-313X</ISSN><JournalIssue CitedMedium="Internet"><Volume>79</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Jul</Month></PubDate></JournalIssue><Title>The Plant journal : for cell and molecular biology</Title><ISOAbbreviation>Plant J</ISOAbbreviation></Journal><ArticleTitle>A universal protocol for the combined isolation of metabolites, DNA, long RNAs, small RNAs, and proteins from plants and microorganisms.</ArticleTitle><Pagination><MedlinePgn>173-80</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/tpj.12546</ELocationID><Abstract><AbstractText>Here, we describe a method for the combined metabolomic, proteomic, transcriptomic and genomic analysis from one single sample as a major step for multilevel data integration strategies in systems biology. While extracting proteins and DNA, this protocol also allows the separation of metabolites into polar and lipid fractions, as well as RNA fractionation into long and small RNAs, thus allowing a broad range of transcriptional studies. The isolated biomolecules are suitable for analysis with different methods that range from electrophoresis and blotting to state-of-the-art procedures based on mass spectrometry (accurate metabolite profiling, shot-gun proteomics) or massive sequencing technologies (transcript analysis). The low amount of starting tissue, its cost-efficiency compared with the utilization of commercial kits, and its performance over a wide range of plant, microbial, and algal species such as Chlamydomonas, Arabidopsis, Populus, or Pinus, makes this method a universal alternative for multiple molecular isolation from plant tissues. </AbstractText><CopyrightInformation>© 2014 The Authors The Plant Journal © 2014 John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Valledor</LastName><ForeName>Luis</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Biology and CESAM, University of Aveiro, Aveiro, Portugal; Global Change Research Centre-Czechglobe, Academy of Sciences of the Czech Republic, Brno, Czech Republic.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Escandón</LastName><ForeName>Mónica</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Meijón</LastName><ForeName>Mónica</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Nukarinen</LastName><ForeName>Ella</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Cañal</LastName><ForeName>María Jesús</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Weckwerth</LastName><ForeName>Wolfram</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>06</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant J</MedlineTA><NlmUniqueID>9207397</NlmUniqueID><ISSNLinking>0960-7412</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D049490" MajorTopicYN="N">Systems Biology</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword 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PubStatus="entrez"><Year>2014</Year><Month>5</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>5</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24804825</ArticleId><ArticleId IdType="doi">10.1111/tpj.12546</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République tchèque</li></country><region><li>Moravie</li></region><settlement><li>Brno</li></settlement></list><tree><noCountry><name sortKey="Ca Al, Maria Jesus" sort="Ca Al, Maria Jesus" uniqKey="Ca Al M" first="María Jesús" last="Ca Al">María Jesús Ca Al</name><name sortKey="Escand N, M Nica" sort="Escand N, M Nica" uniqKey="Escand N M" first="M Nica" last="Escand N">M Nica Escand N</name><name sortKey="Meij N, M Nica" sort="Meij N, M Nica" uniqKey="Meij N M" first="M Nica" last="Meij N">M Nica Meij N</name><name sortKey="Nukarinen, Ella" sort="Nukarinen, Ella" uniqKey="Nukarinen E" first="Ella" last="Nukarinen">Ella Nukarinen</name><name sortKey="Weckwerth, Wolfram" sort="Weckwerth, Wolfram" uniqKey="Weckwerth W" first="Wolfram" last="Weckwerth">Wolfram Weckwerth</name></noCountry><country name="République tchèque"><region name="Moravie"><name sortKey="Valledor, Luis" sort="Valledor, Luis" uniqKey="Valledor L" first="Luis" last="Valledor">Luis Valledor</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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